Modern bioanalytical techniques 02-NMBF

The course concentrates on the developments in analytical methods related to biopolymers (DNA, proteins) exploited in the biotechnology and diagnostics. The aim is to learn the principles of modern methods in bioanalytical chemistry and understanding analytical instruments relative to their range of use and limitations.
LEARNING OUTCOMES.
Upon completion of the course:
(A) Student will know:
��� basics on the structures and functions of biopolymers (DNA, RNA, proteins),
��� properties and diagnostic impact of nucleic acids and proteins,
��� understanding principles of the bioassays used in biomedical diagnosis,
��� understanding sequencing and separation methods of biopolymers,
��� principles and design of instruments in modern bioanalytical chemistry.
(B) Student will demonstrate:
��� the capability to interpret sequencing results of biopolymers,
��� the competence to explain results of gel electrophoresis separations,
��� the ability to design a fluorescent FRET probe,
��� the capacity to write the principles and design of the instruments,
��� the comprehension of the applicability of these instruments,
��� the competence to perform analytical calculations and to analyze the final result,
��� the capability���to carry out bioassay according to the protocol.
(C) Student will:
��� critically evaluate the limitations and future trends, of various bioanalytical techniques,
��� show concern on the quality and assurance of analytical results,
��� obey appropriate safety rules, recognise biohazards, chemical hazards and respond appropriately to laboratory incidents.
Evaluation: the laboratory reports and tests, final exam.