Biophysics of single molecules 04-B-BPC-60-3L

General information about the methods used for the analysis of single macromolecule. Methods which are based on the phenomenon of fluorescence : FCS, FRET and the methods of fluorescence microscopy. Presentation of the methods for the manipulation (AFM, optical and magnetic tweezers) of single macromolecule and the analysis of single ion channels (patch clamp and voltage clam).
Application of the methods for the analysis of denaturation , molecular recognition and internal dynamics of single molecules of proteins and nucleic acids.

Module learning aims

Presentation of the fundamental physic laws, which allow a design of basic instruments for the observation and manipulation of single, biological macromolecule. Presentation of the selected experiments wich are used for the analysis of proteins and nucleic acids.

Major

Biophysics; molecular biophysics

Cycle of studies

1st cycle

Module type

elective

Year of studies (where relevant)

Year 3

Pre-requisites in terms of knowledge, skills and social competences

fundamentals of biochemistry and molecular biology; fundamentals of thermodynamics and quantum physics

Course coordinators

Genowefa Ślósarek

Learning outcomes

One should know the basic phisical laws and phenomena used in the analysis of a single macromolecule; knowledge of the structural transformations of biopolymers (denatyration, aggreration, molecular recognition). Knowledge of the physical description of dynamics and biological function of biopolymers.

Bibliography

Phillips R. i in. “Physical biology of the cell.” Garland Science 2008
Ślósarek G. „Biofizyka molekularna. Zjawiska, instrumenty, modelowanie”, Wydawnictwo Naukowe PWN, Warszawa 2011.
english scientifuc literature

Additional information

Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system:

Description of 04-B-BPC-60-3L in USOSweb