Spectral methods 02-MSPL

Students will understand the interaction of electromagnetic radiation with matter. Students will be able to characterize electromagnetic radiation with wavelength, frequency and wavenumber. Students understand the differences between atomic and molecular spectroscopy. Students will know factors influencing intensity of absoprtion and emission. Students will have the knowledge of various methods of absorption and emission spectroscopy. Students will name basic applications of UV-vis spectrometry. Student can describe the construction of spectrophotometers. Students will understand the principles of taking continuous wavelength and Fourier transform spectra. Students will know how choose spectroscopic methods appropriate for solving an analytical task. Students will comprehend the basic principles of operation of FT NMR instruments, will be able to chose appropriate NMR technique, and will understand how to interpret 1D and 2D NMR spectra. Students will know how to use multinuclear 2D NMR techniques. Students will name various methods of sample ionization used in mass spectrometry. Students will understand basic mass spectrometry techniques. Students will understand the differences between low- and high resolution MS spectra. Students will know how to determine molecular weight of a macromolecule using MS spectrometry. Students will know how to prepare a sample for various spectroscopic techniques. Students can identify an organic compound using a set of IR, MS, and NMR spectra.